Non‐lethal loop‐mediated isothermal amplification assay as a point‐of‐care diagnostics tool for Neoparamoeba perurans , the causative agent of amoebic gill disease

A longitudinal study of amoebic gill disease on a marine Atlantic salmon farm utilising a real-time PCR assay for the detection of Neoparamoeba perurans

Amoebic gill disease (AGD) is a proliferative gill disease of marine cultured Atlantic salmon Salmo salar, with the free-living protozoan Neoparamoeba perurans being the primary aetiological agent. The increased incidence of AGD in recent years presents a significant challenge to the Atlantic salmon farming industry in Europe. In this study, a real-time TaqMan® PCR assay was developed and valid...

A comparison of disease susceptibility and innate immune response between diploid and triploid Atlantic salmon (Salmo salar) siblings following experimental infection with Neoparamoeba perurans, causative agent of amoebic gill disease

Few studies have focussed on the health and immunity of triploid Atlantic salmon and therefore much is still unknown about their response to commercially significant pathogens. This is important if triploid stocks are to be considered for full-scale commercial production. This study aimed to investigate and compare the response of triploid and diploid Atlantic salmon to an experimental challeng...

Evaluation of Non-destructive Molecular Diagnostics for the Detection of Neoparamoeba perurans

1 Fish Health Unit, Marine Institute, Galway, Ireland, Marine and Freshwater Research Centre, Galway Mayo Institute of Technology, Galway, Ireland, CSIRO Agriculture and Food, Integrated Sustainable Aquaculture Production, Hobart, TAS, Australia, 4 Vet Aqua International, Oranmore Business Park, Galway, Ireland, CSIRO Agriculture and Food, Integrated Sustainable Aquaculture Production, St. Luci...

Atlantic salmon (Salmo salar L.) exposed to cultured gill-derived Neoparamoeba branchiphila fail to develop amoebic gill disease (AGD)

Gill-derived Neoparamoeba spp. from Atlantic salmon cause amoebic gill disease (AGD) in naïve recipients. Atlantic salmon were inoculated with clonal gill-derived Neoparamoeba branchiphila that had been cultured in the presence or absence of Atlantic salmon cutaneous mucus. Neoparamoeba branchiphila did not elicit AGD and the supplementation of cultures with cutaneous mucus did not influence vi...

The Study of Gastric Mucosa for Possible Bacterial Causative Agent of Crohn’s Disease